| 1 | Lionsbridge FC | USL League Two | Newport News, VA | 1605 |
| 2 | University of Virginia Cavaliers | NCAA Division I | Charlottesville, VA | 1600 |
| 3 | Bristol Rhythm AFC | NPSL | Bristol, VA | 1571 |
| 4 | Virginia Polytechnic Institute and State University Hokies | NCAA Division I | Blacksburg, VA | 1557 |
| 5 | Virginia Commonwealth University Rams | NCAA Division I | Richmond, VA | 1536 |
| 6 | Virginia Dream FC | UPSL | Reston, VA | 1532 |
| 7 | James Madison University Dukes | NCAA Division I | Harrisonburg, VA | 1531 |
| 8 | Old Dominion University Monarchs | NCAA Division I | Norfolk, VA | 1520 |
| 9 | George Mason University Patriots | NCAA Division I | Fairfax, VA | 1513 |
| 10 | Richmond Kickers | USL League One | Richmond, VA | 1503 |
| 11 | NoVa | APSL | Leesburg, VA | 1489 |
| 12 | Charlottesville Blues FC | USL League Two | Charlottesville, VA | 1479 |
| 13 | Virginia Beach United FC | USL League Two | Virginia Beach, VA | 1472 |
| 14 | Liberty University Flames | NCAA Division I | Lynchburg, VA | 1460 |
| 15 | College of William & Mary Tribe | NCAA Division I | Williamsburg, VA | 1452 |
| 16 | Loudoun United FC 2 | USL League Two | Leesburg, VA | 1451 |
| 17 | Longwood University Lancers | NCAA Division I | Farmville, VA | 1427 |
| 18 | Emory and Henry University Wasps | NCAA Division II | Washington, VA | 1419 |
| 19 | Northern Virginia FC | USL League Two | Leesburg, VA | 1413 |
| 20 | Radford University Highlanders | NCAA Division I | Radford, VA | 1411 |
| 21 | University of Lynchburg Hornets | NCAA Division III | Lynchburg, VA | 1410 |
| 22 | Lynchburg FC | UPSL | Lynchburg, VA | 1406 |
| 23 | Cardinal FC | UPSL | Roanoke, VA | 1401 |
| 24 | Christopher Newport University Captains | NCAA Division III | Newport News, VA | 1400 |
| 25 | Los Toros | UPSL | Reston, VA | 1396 |
| 26 | Hampden–Sydney College Tigers | NCAA Division III | Hampden Sydney, VA | 1394 |
| 27 | Washington and Lee University Generals | NCAA Division III | Lexington, VA | 1385 |
| 28 | Arlington SA | UPSL | Arlington, VA | 1384 |
| 29 | Batten University Marlins | NCAA Division III | Virginia Beach, VA | 1381 |
| 30 | University of Mary Washington Eagles | NCAA Division III | Fredericksburg, VA | 1381 |
| 31 | Grove United | UPSL | Richmond, VA | 1380 |
| 32 | Hill City FC | USL League Two | Lynchburg, VA | 1375 |
| 33 | Doradus FC | UPSL | Springfield, VA | 1373 |
| 34 | Bridgewater College Eagles | NCAA Division III | Bridgewater, VA | 1364 |
| 35 | Richmond United | UPSL | Richmond, VA | 1362 |
| 36 | Virginia Atlantic FC | NPSL | Virginia Beach, VA | 1361 |
| 37 | Roanoke College Maroons | NCAA Division III | Salem, VA | 1361 |
| 38 | Wave Futbol Club | UPSL | Norfolk, VA | 1358 |
| 39 | Virginia Military Institute Keydets | NCAA Division I | Lexington, VA | 1355 |
| 40 | Randolph–Macon College Yellow Jackets | NCAA Division III | Ashland, VA | 1353 |
| 41 | Arlington SA II | UPSL | Arlington, VA | 1350 |
| 42 | Blue Ridge FC II | UPSL | Harrisonburg, VA | 1350 |
| 43 | Cesena FC USA | UPSL | Hendon, VA | 1350 |
| 44 | Club Petrolero | UPSL | Arlington, VA | 1350 |
| 45 | ENFA Sports CD Gouveia SAD | UPSL | McLean, VA | 1350 |
| 46 | Olé Sporting | UPSL | Chesapeake, VA | 1350 |
| 47 | Emory FC | UPSL | Emory, VA | 1349 |
| 48 | Shenandoah University Hornets | NCAA Division III | Winchester, VA | 1347 |
| 49 | Rush United FC | UPSL | Radford, VA | 1341 |
| 50 | Eastern Mennonite University Royals | NCAA Division III | Harrisonburg, VA | 1337 |
| 51 | Alexandria United FC | UPSL | Alexandria, VA | 1334 |
| 52 | Marymount University Saints | NCAA Division III | Arlington, VA | 1334 |
| 53 | Alexandria Reds | UPSL | Alexandria, VA | 1333 |
| 54 | Averett University Cougars | NCAA Division III | Danville, VA | 1331 |
| 55 | Randolph College WildCats | NCAA Division III | Lynchburg, VA | 1329 |
| 56 | Southern Virginia University Knights | NCAA Division III | Buena Vista, VA | 1313 |
| 57 | Blue Ridge FC | UPSL | Harrisonburg, VA | 1308 |
| 58 | Bluefield University Rams | NAIA | Bluefield, VA | 1304 |
| 59 | Skyline Elite SC | UPSL | Charlottesville, VA | 1303 |
| 60 | Summit Valley United | UPSL | Winchester, VA | 1297 |
| 61 | North Stars FC | UPSL | McLean, VA | 1288 |
| 62 | Virginia State University Trojans | NCAA Division II | Chesterfield, VA | 1285 |
| 63 | PW Nova | APSL | VA | 1275 |
| 64 | The St James Football Club | UPSL | Springfield, VA | 1270 |
| 65 | Rush United FC II | UPSL | Radford, VA | 1268 |
| 66 | Mary Baldwin University Fighting Squirrels | NCAA Division III | Staunton, VA | 1242 |
| 67 | Virginia Marauders FC | USL League Two | Winchester, VA | 1094 |